Preservation in a cryobank of Juglans regia L. accession from several populations in the Sairam-Ugam State National Natural Park

Persian walnut (Juglans regia L.) is one of the most economically important nut crops. In Kazakhstan, wild-growing population Juglans registered in south country and protected on territory Sairam-Ugam State National Natural Park. As a result expeditions 2018-2019 136 accessions were described according to descriptors International Plant Genetic Resources Institute, fruits 73 walnuts collected, which are highly diverse terms size, shape weight nuts, kernel yield, color texture shell other parameters. To conserve biodiversity regia, efficient cryopreservation technique has been developed for isolated embryonic axes. After surface disinfection with 0.1% mercuric chloride solution rinsing sterile distilled water, axes desiccated under laminar flow 1 hour moister content 12.1%, placed cryovials, immersed Dewar vessel liquid nitrogen. 72.4% after cryogenic storage, plant recovery was noted Driver-Kuniyuki medium mg/l 6-benzylaminopurine 0.01 indolylbutyric acid. A collection established. For first time, genetic material growing stored bank at -196°C. Key words: L., walnut, cryopreservation, cryobank,